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Simmaron and Advisors’ Publications on CFS/ME


Cytokine network analysis of cerebrospinal fluid in myalgic encephalomyelitis/ chronic fatigue syndrome

M Hornig, G Gottschalk, DL Peterson, KK Knox, AF Schultz, ML Eddy, X Che and WI Lipkin

Molecular Psychiatry advance online publication, 31 March 2015; doi:10.1038/mp.2015.29


“Our results indicate a markedly disturbed immune signature in the cerebrospinal fluid of cases that is consistent with immune activation in the central nervous system, and a shift toward an allergic or T helper type-2 pattern associated with autoimmunity.”

Cytokines in the Cerebrospinal Fluids of Patients with Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

D. Peterson, E. W. Brenu, G. Gottschalk, S. Ramos, T. Nguyen, D. Staines, and S.Marshall-Gradisnik

Mediators of Inflammation, Volume 2015, Article ID 929720,

“This preliminary investigation suggests that perturbations in inflammatory cytokines in the CSF of CFS/ME patients may contribute to the neurological discrepancies observed in CFS/ME.”

Distinct plasma immune signatures in ME/CFS are present early in the course of illness

Mady Hornig, José G. Montoya, Nancy G. Klimas, Susan Levine, Donna Felsenstein, Lucinda Bateman, Daniel L. Peterson, C. Gunnar Gottschalk, Andrew F. Schultz, Xiaoyu Che, Meredith L. Eddy, Anthony L. Komaroff, W. Ian Lipkin

Science Advances 2015;1:e1400121  27 February 2015

“Analyses based on disease duration revealed that early ME/CFS cases had a prominent activation of both pro- and anti-inflammatory cytokines as well as dissociation of intercytokine regulatory networks. We found a stronger correlation of cytokine alterations with illness duration than with measures of illness severity, suggesting that the immunopathology of ME/CFS is not static. These findings have critical implications for discovery of interventional strategies and early diagnosis of ME/CFS.”

Findings from a clinical and laboratory database developed for discovery of pathogenic mechanisms in myalgic encephalomyelitis/chronic fatigue syndrome

N.G. Klimas, G. Ironson, A. Carter, E. Balbin, L. Bateman, D. Felsenstein, S. Levine, D. Peterson, K. Chiu, A. Allen, K. Cunningham, C.G. Gottschalk, M. Fletcher, M. Hornig, C. Canning & A.L. Komaroff

Fatigue: Biomedicine, Health & Behavior Volume 3, Issue 2, 2015

“Potential subgrouping strategies were suggested by these identified symptom clusters: sleep, neurocognitive, autonomic, inflammatory, neuroinflammatory, gastrointestinal and endocrine symptoms.”

Assessment of the Fc receptor CD16A of NK cells of CFS patients for potential to mediate ADCC activity

Isabel Silvestre, Daniel Peterson, Douglas Redelman, Stephen Anderson, Dorothy Hudig

Abstract: Journal of Immunology May 1, 2015 vol.194 (1 Supplement) 52.28


The role of cytokines in the cerebrospinal fluids of patients with Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME)

Sonya Marshall-Gradisnik, Gunnar Gottschalk, Sandra Ramos, Ekua Brenu, Don Staines, Dan Peterson

Abstract: Cytokine, Volume 70, Issue 1, November 2014, SI: 2014 ICIS Abstract Issue

Altered Distribution of Lymphocyte Populations in Chronic Fatigue Syndrome Patients

Isabel Barao, Ph.D., Daniel Peterson, M.D., Dorothy Hudig, Ph.D

Abstract:  Clinical Translational Research Infrastructure Network June 2014

No association found between the detection of either XMRV or polytropic MLV and chronic fatigue syndrome in a blinded, multi-site, prospective study by the establishment and use of the SolveCFS BioBank

David M Irlbeck, Suzanne D Vernon, K Kimberly McCleary, Lucinda Bateman, Nancy G Klimas, Charles W Lapp, Daniel L Peterson, James R Brown, Katja S Remlinger, David A Wilfret and Peter Gerondelis

BMC Research Notes 2014, 7:461


Persistent human herpesvirus-6 infection in patients with an inherited form of the virus.

Journal of Medical Virology, 25 July 2013, doi: 10.1002/jmv.23685

Pantry, S. N., Medveczky, M. M., Arbuckle, J. H., Luka, J., Montoya, J. G., Hu, J., Renne, R., Peterson, D., Pritchett, J. C., Ablashi, D. V. and Medveczky, P. G. (2013)

Abstract: In conclusion, the data presented here document that some individuals with CIHHV-6 are infected persistently with exogenous HHV-6 strains that lead to a wide range of neurological symptoms; the proposed name for this condition is inherited herpesvirus 6 syndrome or IHS.

The adoption of chronic fatigue syndrome/myalgic encephalomyelitis case definitions to assess prevalence: a systematic review

Annals of Epidemiology Volume 23, Issue 6, Pages 371-376, June 2013

Abstract:  Prevalence estimates have been based on several case definitions of chronic fatigue syndrome (CFS). The purpose of this work is to provide a rigorous overview of their application in prevalence research. Read more

Enhanced Gene Expression Following Vaccination in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

International Journal of Clinical Medicine, 2013, 4, 165-170

Abstract:  The objective of this pilot study was to examine the consequences of TIV on cytokine and cytotoxic genes in CFS/ME. Read more.

Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and Parallels with Autoimmune Disorders

“Genes and Autoimmunity – Intracellular Signaling and Microbiome Contribution”, book edited by Spaska Angelova Stanilova, ISBN 978-953-51-1028-6, Published: March 13, 2013

Some unexplained disorders with immune compromises may demonstrate certain characteristics that suggest an autoimmune disorder including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). Read more here.


A Double-Blind, Placebo-Controlled, Randomized, Clinical Trial of the TLR-3 Agonist Rintatolimod in Severe Cases of Chronic Fatigue Syndrome (Ampligen)

DOI: 10.1371/journal.pone.0031334   Published: March 14, 2012

A Phase III prospective, double-blind, randomized, placebo-controlled trial comparing twice weekly IV rintatolimod versus placebo was conducted in 234 subjects with long-standing, debilitating CFS/ME at 12 sites. Rintatolimod produced objective improvement in ET and a reduction in CFS/ME related concomitant medication usage as well as other secondary outcomes. Read more.

[PDF] The Effects of Influenza Vaccination on Immune Function in Patients with Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

Abstract: The purpose of this pilot study was to identify the effects of influenza vaccination on immune function in patients with CFS/ME.

Longitudinal investigation of natural killer cells and cytokines in chronic fatigue syndrome/myalgic encephalomyelitis

Journal of Translational Medicine 2012, 10:88 doi:10.1186/1479-5876-10-88 9 May 2012

Abstract: Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is an etiologically unexplained disorder characterised by irregularities in various aspects of the immunological function. Presently, it is unknown whether these immunological changes remain consistent over time. This study investigates Natural Killer (NK) cell cytotoxic activity, NK cell subsets (CD56brightCD16- and CD56dimCD16+) and cytokines, over the course of a12 month period in patients with CFS/ME…Read more

A Multicenter Blinded Analysis Indicates No Association between Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and either Xenotropic Murine Leukemia Virus-Related Virus or Polytropic Murine Leukemia Virus

mBio 3 (5): eo266-12. doi:10.1128/mBio.oo266-12.Sept 18, 2012

Abstract: The disabling disorder known as chronic fatigue syndrome or myalgic encephalomyelitis (CFS/ME) has been linked in two independent studies to infection with xenotropic murine leukemia virus-related virus (XMRV) and polytropic murine leukemia virus (pMLV). Here we report blinded analysis of peripheral blood from a rigorously characterized, geographically diverse population of 147 patients with CFS/ME and 146 healthy subjects by the investigators describing the original association. This analysis reveals no evidence of either XMRV or pMLV infection.  Read more…

Cytotoxic lymphocyte microRNAs as prospective biomarkers for Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

Journal of Affective Disorders 2012 May 7. 7 May 2012

Abstract:  Immune dysfunction associated with a disease often has a molecular basis. A novel group of molecules known as microRNAs (miRNAs) have been associated with suppression of translational processes involved in cellular development and proliferation, protein secretion, apoptosis, immune function and inflammatory processes. MicroRNAs may be implicated in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME), where immune function is impaired. The objective of this study was to determine the association between miRNAs in cytotoxic cells and CFS/ME… Read more